We analyze unique sets of biomarkers associated with the wound healing process and determine whether experimental therapeutics are likely to enhance normal reepithelialization.
- The complex process of wound healing involves the interaction of many cell types and molecules during overlapping phases to repair skin after injury.
- Wound healing can be investigated ex vivo in tissue explants by engineering wounds and comparing the effects of therapeutic formulations with untreated baseline wounds and wounds treated with preparations known to promote or impair wound healing.
- We use ELISA and multiplex assays that can detect up to 50 proteins at once to determine expression trends in biomarkers associated with wound healing, host antimicrobial activity, and inflammation/chronic wound formation.
- We have panels that measure inflammation-associated protein concentrations, and we are in the process of completing a panel that will predict healthy and rapid wound healing.
- We use fluorescence microscopy for various assays from immunohistochemistry to stains to measure reepithelialization.